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The initial database search identified 451 articles, of which 56 were from PubMed and 395 were from MEDLINE. Data extraction was done on the sample size, animal species, age of humans and animals involved, radiation frequency, exposure setting, SAR, and/or power density values. Studies that used radiation below the range of mobile phone frequency (850 MHz) and Wi-Fi operated at 5 GHz were excluded. Academic journals were retrieved from MEDLINE and PUBMED databases from 1995 to 2020. This review also discusses the factors that influence the total energy absorption of RF-EMR emitted by wireless devices and their importance. The loss of the germ cells will ultimately affect the hormonal balance as it is one of the important components in the hypothalamus-pituitary-gonadal (HPG) axis.
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In the same study, the exposed group had a considerable decrease in LH levels but no remarkable differences in FSH levels. This specialized laboratory equipment was developed to ensure the consistency of RF-EMR exposure. In comparison, Saygin et al. (2016) used a rotating carousel with 12 rats in a cylindrical restraint at the same time. Kumar et al. (2011) had lined the exposure cage with radar-absorbing material and then positioned the cage vertically to irradiate all of the animals uniformly at a single power level. Differences in SAR values, exposure duration, [39.171.252.63](http://39.171.252.63:3000/carolinedunkel) and exposure systems used in the studies could explain the contradictory findings. In comparison, two studies (33%) found no significant differences in FSH and LH levels in the exposed group (Ozguner et al., 2004; Çetkin et al., 2017). These findings are consistent with the study by Gevrek et al. (2017), which also reported a significant decrease in LH serum levels in the RF-EMR-exposed group.
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A study used a dual-band 900/1,800 mobile phone as RF-EMR source (Oyewopo et al., 2017). In the studies that used mobile phones, the frequency applied includes 850–950 (Kesari and Behari, 2012; Zang et al., 2016; Çetkin et al., 2017) and 1,800–1,850 MHz (Ribeiro et al., 2007; Shahin et al., 2018). The study design, including the age of the animals, the frequency of radiation, the type of radiation generator used, the distance between cages and generator, and the duration of exposure, differs in several ways. Finally, another two articles were excluded after full-text review because both articles were retracted due to inadequate evidence [best place to buy testosterone](http://183.6.47.107:30000/edith712244122) justify the accuracy of the data presented and the overlapping data and figures between these two articles.
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